XIAO Jinbo2
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Objective This study aimed to systematically assess the epidemiological characteristics of enteroviruses(EV) among healthy children in the Xizang Autonomous Region,China during 2023 – 2024, providing a basis for regional viral surveillance and disease prevention.Methods A total of 456 fecal samples from healthy children were analyzed, and EV screening was performed using real-time reverse-transcription polymerase chain reaction(real-time RT-PCR). The VP1 region of EV-positive samples was amplified and sequenced by RT-PCR, followed by molecular typing and phylogenetic analysis of the obtained sequences.Results The overall EV detection rate was 21.71%(99/456). Detected viruses were classified into Enterovirus A(EV-A), Enterovirus B(EV-B), and Enterovirus C(EV-C), among which EV-B predominated, accounting for(54.55%, 54/99) of positive samples. The most frequently detected serotypes were Coxsackievirus A4(CVA4, 26/99, 26.26%), Coxsackievirus B5(CVB5, 23/99, 23.23%), Coxsackievirus B4(CVB4, 14/99, 14.14%), Coxsackievirus B2(CVB2, 10/99, 10.10%), and poliovirus type 3(PV3, 9/99, 9.09%). EV infections were mainly observed in children aged 3 – 5 years, with no statistically significant difference between sexes. Regional analysis indicated that Lhasa exhibited the greatest diversity of EV serotypes, while CVA4 and CVB serotypes predominated in Shigatse and Ngari, and CVB5 was most prevalent in Shannan. Phylogenetic analysis showed that CVB2, CVB4, and CVB5 isolates from the Xizang Autonomous Region all belonged to genotype D of their respective serotypes and were closely related to contemporary strains circulating both domestically and internationally. Conclusion This study delineates the epidemiological profile of EVs among healthy children in the Xizang Autonomous Region during 2023– 2024. When combined with surveillance data from 2020 – 2022, a notable increase in the circulation intensity of Coxsackievirus B was observed, underscoring the need for sustained continuous surveillance and targeted prevention and control measures for CVB in this region.
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Basic Information:
DOI：10.13242/j.cnki.bingduxuebao.250371
China Classification Code:R512.5
Citation Information:
[1]SHAO Kexin,HONG Mei,XIAO Mengyi ,et al.Enterovirus Surveillance among Healthy Individuals in Xizang Autonomous Region, China, 2023–2024: Increasing Circulation of Coxsackievirus B[J].Chinese Journal of Virology,2026,42(01):51-59.DOI:10.13242/j.cnki.bingduxuebao.250371.
Fund Information:
国家疾病预防控制局公共卫生人才培养支持项目（项目号：GJJKJ-2024-ZY）; 传染病溯源预警与智能决策全国重点实验室（NITFID）资助项目（项目号：ZDGWNLJS25-36）,题目：未知及新发病原体监测及预测预警技术研究; 国家重点研发计划项目（项目号：2021YFC2302003）,题目：病毒监测网络数据标准及数据平台建设~~