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Subgroup J avian leukosis viruses (ALV J) were isolated from two broiler breeder farms with suspected diseased chickens and two commercial broiler flocks without clinical symptoms by inoculating the samples into chicken embryo fibroblast cells and PCR amplification of the infected CEF genomic DNA. In the indirect fluorescence assay (IFA) with ALV J specific monoclonal antibody JE9, 2 strains, SD9901 and SD9902 from breeder with suspected lesions, were strongly positive, another 2 strains, YZ9901 and YZ9902 from commercial broilers without clinical symptoms gave weak reactions. The genomes of strains YZ9901 and SD9902 were partially sequenced and the results indicated that their gp85 had 89%-93% identity in the amino acid level with ALV J prototype HPRS 103 and American strain ADOL HCl. The amino acid identity among themselves was 92%. The 3' noncoding LTR region had 95%-97% identity in the nucleotide level with ALV J prototype strain HPRS 103. But the Chinese strains had a 139 base deletion mutation in their E elements nearby the 3' LTR region and got an insertion of 11 base fragment instead.
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Basic Information:
DOI：10.13242/j.cnki.bingduxuebao.001274
China Classification Code:S852.65
Citation Information:
[1]DU Yan 1, CUI Zhi zhong 1,2 , QIN Ai jian 1, Silva R. F. 3, Lee L. F. 3 (1.Department of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China, 2. College of Animal Science, Shandong Agricultural University, Taian 271018, China, 3..Isolation of Subgroup J Avian Leukosis Viruses and Their Partial Sequence Comparison[J].Chinese Journal of Virology,2000(04):341-346.DOI:10.13242/j.cnki.bingduxuebao.001274.
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国家自然科学基金生物学部主任基金!项目资助 (30 0 40 0 11)